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Herbal Synergic effect’s study
It has been made a scientific study to demonstrate the synergic effect of the 3 herbs that compose our product. For this reason it was determinated the percentage of cell proliferation inhibition of mother tincture and in each tincture of herbal extract separated, in different dilutions. In the table below the results are shown as percentage of inhibition of lines cells growth up BW 5147 at differents dilutions of mother tincture (moisture of 3 herbs).
Dilution |
BW5147 |
I.E. |
% Inhib |
1/25 |
0.03 |
97.4 |
1/50 |
0.045 |
95.3 |
1/100 |
0.11 |
88.7 |
1/250 |
0.26 |
74.0 |
1/500 |
0.48 |
51.7 |
1/1000 |
0.70 |
29.6 |
1/2000 |
0.88 |
12.4 |
1/4000 |
0.87 |
13.0 |
TABLE I: Percentage of growth up inhibition of cell lines BW 5147 at differents dilutions of mother tincture.
INDIVIDUAL EXTRACTS’S TINCTURES:
Below it is shown Table II: “ Inhibitory effect of the tinctures of 3 herbs alone”.
Dilution |
PLANTAGO (P) |
CARQUEJA (C) |
ROMERO (R) |
I.E. |
% Inhib |
I.E. |
% Inhib |
I.E. |
% Inhib |
1/25 |
0.75 |
24.9 |
0.50 |
49.7 |
0.12 |
88 |
1/50 |
0.73 |
26.8 |
0.58 |
42 |
0.21 |
78.5 |
1/100 |
0.98 |
1.9 |
0.85 |
14.5 |
0.41 |
58 |
1/500 |
1.0 |
0 |
1.04 |
0 |
1.12 |
0 |
TABLR II: Inhibitory effect ofthe tinctures of 3 herbs alone.
In case of individual extracts’s tinctures was proved a notorious percentage of cell proliferation inhibition of each herb alone, withimportant percentage of Rosmarinus that showed an 88 % of inhibition with a 1/25 dilution.
Comparating inhibitories effects to equal dilutions is proved that in all of the case is bigger the effect of the mixture than the each one of 3 herbs alone, marking clearly the mentioned synergic effect in the dilution 1/500 point where the extracts each one alone don’t present any inhibition, in contraposition with the mixture that this dilution presents an inhibition percentage of 51.7 % .
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